Coronavirus illness 2019 (COVID-19) is a newly rising human infectious illness attributable to extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2, beforehand known as 2019-nCoV). Based mostly on the speedy enhance within the price of human an infection, the World Well being Group (WHO) has categorized the COVID-19 outbreak as a pandemic. As a result of no particular medicine or vaccines for COVID-19 are but accessible, early analysis and administration are essential for holding the outbreak. Right here, we report a field-effect transistor (FET)-based biosensing machine for detecting SARS-CoV-2 in scientific samples. The sensor was produced by coating graphene sheets of the FET with a particular antibody in opposition to SARS-CoV-2 spike protein. The efficiency of the sensor was decided utilizing antigen protein, cultured virus, and nasopharyngeal swab specimens from COVID-19 sufferers. Our FET machine might detect the SARS-CoV-2 spike protein at concentrations of 1 fg/mL in phosphate-buffered saline and 100 fg/mL scientific transport medium. As well as, the FET sensor efficiently detected SARS-CoV-2 in tradition medium (restrict of detection [LOD]: 1.6 × 101 pfu/mL) and scientific samples (LOD: 2.42 × 102 copies/mL). Thus, we now have efficiently fabricated a promising FET biosensor for SARS-CoV-2; our machine is a extremely delicate immunological diagnostic technique for COVID-19 that requires no pattern pretreatment or labeling.
Key phrases: 2019-nCoV; COVID-19; FET; SARS-CoV-2; biosensor.